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51613

TAQ DNA Polymerase (5U/μl) (w/6X Buffer, w/dNTP)


Part E
Storage : -20 °C (Blue/Dry Ice)
Shelf Life : 24 Months
HSN Code : 38220090
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
Product Datasheet
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Specifications
 
Appearance (Form)Liquid
Appearance (Colour)Colourless
Appearance (Clarity)Clear
Concentration5U/μl
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C
Amplification capabilityUp to 5 kb DNA fragment
Absence of nucleasesNo obvious degradation was seen after incubation at room temperature for 1 week
DNA contaminationNo Genomic or Plasmid DNA contamination
Notes
  Description:
Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

Applications:
PCR amplification of DNA fragments as long as 5 kb
DNA labeling.
DNA sequencing.
PCR for cloning.

Includes:
500 Units
• TAQ Polymerase (5U/μl) - 100 μl
• 10X PCR Buffer (MgCl2 plus) - 1.25 ml
• 6x Loading Buffer - 1.0 ml
• dNTP mix (each 2.5mM) - 1.0 ml

1000 Units
• TAQ Polymerase (5U/μl) - 200 μl
• 10X PCR Buffer (MgCl2 plus) - 1.25ml x 2
• 6x Loading Buffer - 1.0ml
• dNTP mix (each 2.5mM) - 1.0 ml

5000 Units
• TAQ Polymerase (5U/μl) - 200 μl x5
• 10X PCR Buffer (MgCl2 plus) - 1.25ml x 10
• 6x Loading Buffer - 1.0ml x 5
• dNTP mix (each2.5mM) - 1.0 ml x 5
 
Available Packages
 
Size Price  
500 Units
INR 2921.00  
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1000 Units
INR 4867.00  
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5000 Units
INR 22171.00  
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Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
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