Pack Size

Price

100 Gms
14.48
500 Gms
56.59

Prices are indicative and are subject to change

Appearance (Colour)

Beige

Appearance (Form)

Free,flowing,homogeneous powder.

Solubility

43.41 gms/lit.

pH (25°C)

7.4 -7.8

Gel strength

Firm,comparable with 1.5% agar gel

Prepared Medium Appearance after autoclaving (Colour)

Light amber

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Cultural Response: Inoculate and incubate at 37° C for 18-24hrs

with addition of blood and supplements under anaerobic conditions.

Organism

Bacteroides fragilis ATCC 25285

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Organism

Clostridium perfringens ATCC 12924

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Organism

Streptococcus pyogenes ATCC 19615

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Organism

Escherichia coli ATCC 25922

Inoculum

>=10³
Growth : Inhibited

Applications :


Used for the enumeration of various aerobic and anaerobic bacterial species present in the gastrointestinal tract.

Composition :


Ingredients

gm/lt.

Enzymatic Digest of Casein

5.67

Enzymatic Digest of Animal Tissue

5.00

Soya peptone

1.00

Yeast extract

5.00

Dextrose

5.83

Sodium chloride

1.67

Tris (hydroxymethyl) aminomethane

3.00

Hemin

0.01

L-Cystine

0.40

Dipotassium hydrogen phosphate

0.83

Agar

15.00

Directions :


1. Suspend 43.41 gms of powder in 950 ml distilled/purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 15 lbs pressure at 121 °C for 15 minutes.
4. Cool to 45-50°C.
5. If desired,add 5.0%v/v sterile defibrinated sheep blood.
6. If desired,add rehydrated contents of Vitamin k1 supplement (34326) and CNA supplement (64078).
7. Mix well and dispense as desired.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Product Datasheet

Sample COA

Certificate Of Analysis