Pack Size

Price

100 Gms
14.48
500 Gms
61.85

Prices are indicative and are subject to change

Appearance (Colour)

Light grey

Appearance (Form)

Free flowing, homogeneous powder

Solubility

47.02 gm/liter

Solubility before autoclaving (Clarity)

Slightly opalescent

Gel strength (1.5% gel)

Firm, comparable with 1.45% agar gel

pH (25°C)

7.4 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Purple

Cultural Response

Inoculate and incubate at 35 ± 2°C for 18-48 hours

Organism

Staphylococcus aureus ATCC 25923

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=70.0%
Mannitol fermentation : Positive reaction, yellow color
Coagulase production : positive reaction, opaque zone around the colonies

Organism

Staphylococcus epidermidis ATCC 12228

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=70.0%
Mannitol fermentation : Negative reaction,purple color
Coagulase production : Negative reaction, no formation of opaque zone around the colonies -

Applications :


For the cultivation, differentiation of Staphylococcus aureus from other Staphylococcus species, based on coagulase production and mannitol fermentation.

Composition :


Ingredients

gms/lt.

Brain heart infusion

5.00

Tryptone

10.50

Soyatone

3.50

Sodium chloride

3.50

Mannitol

10.00

Bromocresol Purple

0.02

Agar

14.50

Directions :


1. Add 47.02 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C and add 7-15% sterile, rabbit plasma to the basal medium.
5. Mix well and pour in sterile petri plates.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

500 Gms

Plastic Bottle

Product Datasheet

Certificate Of Analysis