Pack Size

Price

100 Gms
25.00
500 Gms
82.91

Prices are indicative and are subject to change

Appearance (Colour)

White to off-white

Appearance (Form)

Free flowing, homogeneous powder

Solubility

56.40 gm/liter

Solubility

Colorless,clear solution with no significane precipitate

pH (25°C)

6.8 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear with no significant precipitate

Prepared Medium Appearance after autoclaving (Colour)

Colourless

Cultural Response

Prepare the medium and dilute to 1X. Supplement with glucose as given in the directions. Incubate at 35 ± 2°C for 18-48 hours.

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : Good

Organism

Escherichia coli ATCC 23724

Inoculum (cfu) 10-100

Growth : Good

Applications :


For the cultivation of recombinant strains of Escherichia coli.

Composition :


Ingredients

gm/lt.

Disodium phosphate, anhydrous

33.90

Sodium chloride

2.50

Monopotassium phosphate

15.00

Ammonium chloride

5.00

Directions :


1. Add 56.40 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Autoclave at 121°C at 15 psi for 15 minutes.
4. To prepare M9 Minimal salts medium, add 200 ml M9 Minimal Salts, 5X to 750 ml sterile distilled water.
5. Cool to 45-50°C. Adjust final volume to 1.0 liter.
6. Aseptically add 20 ml of filter sterilized 20% glucose solution and, if desired 0.1 ml sterile 1.0 M Calcium chloride solution. Mix well.
7. If desired, supplement with amino acids.

Storage

8 to 25°C (Cool & Dry Area)

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

500 Gms

Plastic Bottle

Product Datasheet

Sample COA

Certificate Of Analysis