Pack Size

Price

100 Gms
11.84
500 Gms
36.85

Prices are indicative and are subject to change

Appearance (Colour)

Biege

Appearance (Form)

Free flowing, homogeneous powder

Solubility

82.05 gm/liter

Solubility before autoclaving (Clarity)

Opalescent with heavy white precipitate.

pH (25°C)

7.8 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Opalescent with heavy white precipitate.

Prepared Medium Appearance after autoclaving (Colour)

Light green

Cultural Response

Inoculate and incubate the tubes at 37 ± 2°C for 18-24 hrs. Cultural response observed when subcultured on Soyabean Casein Digest Agar (SCDA) , after incubation at 42 - 44°C for 18-24 hrs.

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : None - poor, Growth on SCDA : None-poor

Organism

Proteus vulgaris ATCC 13315

Inoculum (cfu) 10-100

Growth : None - poor, Growth on SCDA : None-poor

Organism

Salmonella typhimurium ATCC 14028

Inoculum (cfu) 10-100

Growth : Good, Growth on SCDA : Good

Organism

Salmonella paratyphi B ATCC 8759

Inoculum (cfu) 10-100

Growth : Good, Growth on SCDA : Good

Organism

Shigella flexneri ATCC 12022

Inoculum

Growth : Inhibited, Growth on SCDA : Inhibited

Applications :


An improved enrichment medium for the isolation of Salmonellae and the suppression of Proteus species.

Composition :


Ingredients

gm/lt.

Sodium thiosulphate

40.7

Calcium carbonate

25.0

Sodium chloride

2.30

Oxbile

4.75

Soyatone

2.30

Casein, enzymatic hydrolysate

7.0

Directions :


1. Add 82.05 gm powder to 1000 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave.
4. Cool to 45-500C.
5. Aseptically, add 19.0 ml of Iodine solution (in 20gm iodine add 25gm potassium iodide in 100ml sterile distilled water.) and 9.5ml of 0.1% Brilliant Green solution.
6. If desired ,add Novabiocin supplement (18352).
7. Mix well to disperse precipitate uniformly.
8. Dispense into sterile test tubes.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Safety Datasheet

Product Datasheet

Sample COA

Certificate Of Analysis