Pack Size

Price

100 Gms
13.16
500 Gms
55.27

Prices are indicative and are subject to change

Appearance (Colour)

Cream, free flowing, homogeneous powder

Solubility

28.7 gm/500ml

Solubility before autoclaving (Clarity)

Clear solution with very slight precipitate

pH (25°C)

7.2 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear solution with very slight precipitate

Prepared Medium Appearance after autoclaving (Colour)

Medium amber to amber (without addition of supplement)
Medium amber to yellow clear solution with very slight precipitate (after addition of supplement)

Cultural Response

Inoculate and incubate at 35 ± 2°C for 24-48 hrs

Organism

Listeria monocytogenes
ATCC 19118

Inoculum (cfu) 10-100

Growth : Good,
Esculin Hydrolysis : +( postive, blackening of medium)

Organism

Pseudomonas aeruginosa
ATCC 27853

Inoculum (cfu) 10-100

>=103
Growth : Inhibited
Esculin Hydrolysis : -(negative, no blackening of medium)

Organism

Listeria monocytogenes
ATCC 19117

Inoculum (cfu) 10-100

Growth : Good,
Esculin Hydrolysis : +( postive, blackening of medium)

Organism

Escherichia coli
ATCC 25922

Inoculum

>=103
Growth : Inhibited
Esculin Hydrolysis : -(negative, no blackening of medium)

Applications :


Recommended selective, enrichment medium for the isolation of Listeria species.

Composition :


Ingredients

gm/lt.

Proteose peptone

5.00

Tryptone

5.00

Beef extract(ex.buffalo)

5.00

Yeast extract

5.00

Sodium chloride

20.00

Disodium hydrogen phosphate

12.00

Potassium dihydrogen phosphate

1.35

Esculin

1.00

Lithium chloride

3.00

Directions :


1. Add 28.7 gm powder to 500ml distilled/purified water.
2. Mix well to dissolve completely.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C & add aseptically add the contents of 1 vial of Fraser Selective Supplement (52071) or as desired.

Note : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

500 Gms

Plastic Bottle

Safety Datasheet

Product Datasheet

Sample COA

Certificate Of Analysis