Pack Size

Price

100 Gms
22.37
500 Gms
69.75

Prices are indicative and are subject to change

Appearance (Colour)

Light yellow to light green

Appearance (Form)

Free flowing, homogeneous powder

Solubility

22.00 gm/liter

Solubility before autoclaving (Clarity)

Clear to slightly opalescent

Gel strength (1.5% gel)

Firm, comparable with 1.54% agar gel

pH (25°C)

7.2 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Light purple

Cultural Response

Inoculate and incubate at 37°C ± 2°C for 18 - 24 hours.

Organism

Bacillus subtilis ATCC 6633

Inoculum (cfu) 10-100

Growth : Good
Recovery : =>70%
Colour : Yellow

Organism

Bacillus cereus ATCC 10876

Inoculum (cfu) 10-100

Growth : Good
Recovery : =>70%
Colour : Colourless

Applications :


Used for the differentiation between Bacillus cereus and Bacillus subtilis based on mannitol fermentation.

Composition :


Ingredients

gms/lt.

Mannitol

5.00

Yeast autolysate

0.20

Monohydrogen ammonium phosphate

1.00

Potassium chloride

0.20

Magnesium sulphate

0.20

Bromocresol purple

0.0075

Agar

15.40

Directions :


1. Add 22.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38151290 (GST 18%)

500 Gms

38151290 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

Product Datasheet

Sample COA

Certificate Of Analysis