SRL

70637

Lactose TTC Agar (w/ Tergitol 7) (I)

(Lactose Triphenyltetrazolium Chloride Agar)

Part D

Pack Size

Price

100 Gms
13.16
500 Gms
48.69

Prices are indicative and are subject to change

Appearance (Colour)

Yellow with greenish tinge

Appearance (Form)

Free flowing, homogeneous powder

Solubility

56.15 gms/liter

Solubility before autoclaving (Clarity)

Clear to slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

7.2 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Green

Cultural Response

Inoculate and incubate at 37°C for 18 - 24 hours

Organism

Escherichia coli
ATCC 25922

Inoculum (cfu) 10-100

Growth : Good
Colony colour : orange yellow with yellow corona

Organism

Pseudomonas aeruginosa
ATCC 27853

Inoculum (cfu) 10-100

Growth : Good
Colony colour : red with blue corona

Organism

Shigella flexneri
ATCC 12022

Inoculum (cfu) 10-100

Growth : Good
Colony colour : red with blue corona

Organism

Salmonella typhimurium
ATCC 14028

Inoculum (cfu) 10-100

Growth : Good
Colony colour : red with blue corona

Organism

Proteus mirabilis
ATCC 25933

Inoculum (cfu) 10-100

Growth : Good
Colony colour : red with blue corona

Organism

Staphylococcus aureus
ATCC 25923

Inoculum

Growth : Inhibited

Applications :


Selective differential medium for the detection and enumeration of Escherichia coli and coliform bacteria in water using the membrane filtration method.

Composition :


Ingredients

gms/lt.

Lactose

20.00

Peptone

10.00

Yeast extract

6.00

Meat extract

5.00

Tergitol-7

0.10

Bromothymol Blue

0.05

Agar

15.00

Directions :


1. Add 56.15 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C and aseptically add 2.5 ml of 1% TTC solution (55052). Mix well.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

500 Gms

Plastic Bottle

Product Datasheet

Sample COA

Certificate Of Analysis