Pack Size

Price

100 Gms
11.84
500 Gms
42.11

Prices are indicative and are subject to change

Appearance (Colour)

Biege with greyish tinge

Appearance (Form)

Free flowing, homogeneous powder

Solubility

34.56 gm/liter

Solubility before autoclaving (Clarity)

Clear to slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

6.7 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Purple

Cultural Response

Inoculate and incubate at 37°C for 18 - 24 hours

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth       Slant           Butt               H2S
Good           K                  K                    -

Organism

Salmonella typhimurium ATCC 14028

Inoculum (cfu) 10-100

Growth       Slant           Butt               H2S
Good           K                  K                    +

Organism

Proteus mirabilis ATCC 25933

Inoculum (cfu) 10-100

Growth       Slant           Butt               H2S
Good           A                  R                    +

Organism

Shigella flexneri ATCC 12022

Inoculum (cfu) 10-100

Growth       Slant           Butt               H2S
Good           A                  K                    -

Key

A = acidic, yellow; K = alkaline, purple, no colour change; R = deep red, lysine deamination; H2S = (+) blackening of medium; H2S = (-) no blackening of medium.

Applications :


For the differentiation of enteric organisms, especially Salmonella, based upon lysine decarboxylation/ deamination and H2S production.

Composition :


Ingredients

gm/lt.

L-Lysine

10.00

Pancreatic digest of gelatin

5.00

Yeast extract

3.00

Dextrose

1.00

Ferric ammonium citrate

0.50

Sodium thiosulphate

0.04

Bromocresol Purple

0.02

Agar

15.00

Directions :


1. Add 34.56 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into test tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Product Datasheet

Sample COA

Certificate Of Analysis