SRL

97676

B.C.P. - D.C.L.S. Agar

(Bromocresol Purple-Deoxycholic Acid-Citrate-Lactose-Sucrose Agar)

Part D

Pack Size

Price

100 Gms
17.11
500 Gms
63.17

Prices are indicative and are subject to change

Appearance (Colour)

Beige

Appearance (Form)

Free flowing, homogeneous powder

Solubility

67.52 gm/liter

Solubility before boiling (clarity)

Clear to slightly opalescent

Gel strength (1.5% gel)

Firm, comparable with 1.5% agar gel

pH (25°C)

7.2 ± 0.2

Prepared medium appearance after boiling (clarity)

Clear to slightly opalescent

Prepared medium appearance after boiling (colour)

Purple coloured

Cultural Response

Inoculate and incubate at 35-37°C for 18-24 hours

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : None-poor
Recovery rate : <=10.0%
Colour of colony : yellow

Organism

Proteus mirabilis ATCC 12453

Inoculum (cfu) 10-100

Growth : None-poor
Recovery rate : <=10.0%
Colour of colony : colourless

Organism

Salmonella enteritidis ATCC 13076

Inoculum (cfu) 10-100

Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies

Organism

Shigella dysenteriae ATCC 13313

Inoculum (cfu) 10-100

Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies

Organism

Shigella flexneri ATCC 12022

Inoculum (cfu) 10-100

Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies

Organism

Salmonella typhimurium ATCC 14028

Inoculum (cfu) 10-100

Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies

Organism

Shigella sonnei ATCC 25931

Inoculum (cfu) 10-100

Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies

Applications :


Used for the selective isolation of Salmonella and Shigella species.

Composition :


Ingredients

gm/lt.

Peptic digest of animal tissue

5.00

Yeast extract

3.00

Beef extract(ex.buffalo)

3.00

Pancreatic digest of casein

5.00

Lactose

7.50

Sucrose

7.50

Sodium citrate

10.00

Sodium chloride

5.00

Sodium thiosulphate

5.00

Sodium deoxycholate

2.50

Bromocresol Purple

0.02

Agar

14.00

Directions :


1. Add 67.52 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Cool to 45- 50°C.
4. Do not autoclave.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

Product Datasheet

Sample COA

Certificate Of Analysis