Pack Size

Price

100 Gms
23.69
500 Gms
101.33

Prices are indicative and are subject to change

Appearance (Colour)

Cream to yellow

Appearance (Form)

Free flowing, homogeneous powder

Solubility

69.05 gm/liter

Solubility before autoclaving (Clarity)

Clear to slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

7.2 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Slightly opalescent gel

Prepared Medium Appearance after autoclaving (Colour)

Yellow

Cultural Response

Inoculate and incubate at 30-37°C for 24-48 hours

Organism

Listeria monocytogenes ATCC 19118

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >= 50.0%
Colony colour : Blue/green plus halo

Organism

Listeria innocua ATCC 33090

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%
Colony colour : Blue/green no halo

Organism

Escherichia coli ATCC 25922

Inoculum

Growth : Inhibited

Applications :


A selective and differential chromogenic medium recommended for rapid and direct identification of listeria species.

Principle :


Enzymatic digest of animal tissue, enzymatic digest of casein and Yeast extract provides the nitrogenous sources of nutrients. Lithium chloride inhibits the non-targeted organisms. The combination of phospholipase enzyme reaction and chromogenic substrate aids in easy differentiation of Listeria monocytogenes (blue colonies surrounded by opaque halo), from other Listeria species.

Composition :


Ingredients

gm/lt.

Enzymatic digest of animal tissue

18.00

Enzymatic digest of casein

6.00

Sodium pyruvate

2.00

Glucose

2.00

Magnesium glycerophosphate

1.00

Magnesium sulphate anhydrous

0.50

Sodium chloride

5.00

Yeast extract

10.00

Lithium Chloride

10.00

Disodium hydrogen phosphate anhydrous

2.50

Chromogenic mixture

0.50

Agar

12.00

Directions :


1. Add 34.50 gm powder to 480ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes. If desired, aseptically add 1 vial of listeria selective supplement, mix well.

Storage

2 to 8°C (Refrigerate)

Shelf Life

24 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

500 Gms

Plastic Bottle

Safety Datasheet

Product Datasheet

Sample COA

Certificate Of Analysis