Pack Size

Price

100 Gms
55.27
500 Gms
211.88

Prices are indicative and are subject to change

Appearance (Colour)

Cream

Appearance (Form)

Free flowing, homogeneous powder

Solubility

55.83 gm/liter

Solubility before autoclaving (Clarity)

Clear to slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

6.8 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Light to medium amber

Cultural Response

Inoculate and incubate at 37 ± 2°C for 18 - 24 hours

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=70.0%
Colony characteristics : Blue-dark violet colonies

Organism

Escherichia coli ATCC 8739

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=70.0%
Colony characteristics : Blue-dark violet colonies

Organism

Salmonella enteritidis ATCC 13076

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=70.0%
Colony characteristics : Colourless colonies

Organism

Enterococcus faecalis ATCC 29212

Inoculum

Growth : Inhibited

Applications :


Differential medium recommended for presumptive identification of Escherichia coli and other coliforms in food and environmental samples.

Principle :


Peptone and Yeast extract provides the nitrogenous sources of nutrition and other essential growth nutrients. Disodium phosphate and Potassium dihydrogen phosphate serves as buffering agents. Lactose is the fermentable carbohydrate source and Sodium chloride
maintains the osmotic equilibrium. Glucuronidase present in E coli, cleaves one of the chromogenic substrates to yield blue to violet colonies. The other chromogenic substrate is cleaved by majority of coliforms (due to activity of enzyme galactosidase) resulting in formation of rose-pink coloured colonies.

Composition :


Ingredients

gm/lt.

Peptone

5.00

Yeast extract

3.00

Lactose

2.50

Disodium hydrogen phosphate

3.50

Potassium dihydrogen phosphate

1.50

Sodium chloride

5.00

Chromogenic mixture

14.50

Agar

15.00

Directions :


1. Add 55.83 gm powder to 1.0 liter distilled/purified water.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.

Storage

2 to 8°C (Refrigerate)

Shelf Life

24 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

Safety Datasheet

Product Datasheet

Certificate Of Analysis