Prices are indicative and are subject to change
Amplification with good yield is seen with the template and primers in the kit
Protocol given with kit shows good amplification with amplicons of the sizes ranging from either 600 to 900bp.
Polymerase Chain Reaction (PCR) is an important tool in Biotechnology. PCR rapidly amplifies a specific region of a single DNA molecule in vitro to yield sufficient quantities that can be cloned, sequenced, analyzed by restriction mapping etc. In this kit the gene for E.coli pyrophosphatase is amplified from its template DNA using specific primers in 30 cycles of operation.
Components | 10 Experiments | 25 Experiments |
---|---|---|
Control (with 36 mcl of PCR master mix) | 12 vials | 28 vials |
Test (with 1 mcl template) | 12 vials | 28 vials |
Taq Polymerase (5U/mcl) | 10 mcl | 20 mcl |
Enzyme Diluent Buffer | 50 mcl | 150 mcl |
6 X Gel loading Dye | 120 mcl | 250 mcl |
Agarose | 0.75 g | 2.5 g |
Ethidium Bromide | 50 mcl | 100 mcl |
50 X TAE buffer | 25 ml | 50 ml |
Sterile Water | 100 mcl | 250 mcl |
DNA marker | 60 mcl | 100 mcl |
-20 °C (Blue/Dry Ice)
12 Months
Not Regulated for Transport (Non-Haz)
38229090 (GST 12%)
38229090 (GST 12%)