Description :

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA
strand.

This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.

The enzyme has increased thermal stability, that allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ to 5’ exonuclease and reduced RNaseH activity, that improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNa synthesis up to 10kb long.

Applications :

PCR,
real-time PCR
second strand cDNA synthesis
DNA labeling
Analysis of RNA by primer extension

Includes :

5000U
· M-MLV (Recombinant) (200U/μl ) - 25 μl
· Reaction Buffer (10X) - 50 μl

10000U
· M-MLV (Recombinant) (200U/μl ) - 50 μl
· Reaction Buffer (10X) - 100 μl

HSN Code :