Pack Size

Price

1000 Units
105.28
5000 Units
513.24

Prices are indicative and are subject to change

Appearance of Solution

Liquid

Appearance (Colour)

Colourless

Appearance (Clarity)

Clear

Absence of nucleases

Free of endonuclease, exonuclease, RNase and E.coli RNA polymerase activities

Assay

Functional assay with high yield in T7 RNA transcription

Purity

>95% by SDS PAGE

Description :


T7 RNA polymerase is a monomeric bacteriophage encoded DNA directed RNA polymerase which catalyzes the formation of RNA in the 5`-3` direction. In the process of initiation of transcription T7 recognizes a specific promoter sequence, the T7 promoter. T7 consists of 883 amino acids and has a molecular weight of 99 kDa.

Application :


Radiolabeled RNA probe preparation
RNA generation for in vitro translation
RNA generation for studies of RNA structure, processing and catalysis
Expression control via anti-sense RNA
Vaccine production
In situ hybridization

Unit Definition:

One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of rCTP into acid-insoluble product in 1 hour at 37°C in a total volume of 100μl.

Storage

-20 °C (Blue/Dry Ice)

Shelf Life

24 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


1000 Units

38220090 (GST 12%)

5000 Units

38220090 (GST 12%)

Product Datasheet

Sample COA

Certificate Of Analysis