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One unit is defined as the amount of enzyme required to digest 1 µg of lambda DNA in 1 hour at 37 °C in 50 µL of assay buffer.
Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C.
After 160-fold overdigestion (10 U/µg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity.
EcoRV is an endonuclease enzyme isolated from strains of Escherichia.coli J62plg 74, and is part of the restriction modification system. It is also known as Eco321.
In molecular biology it is used as a restriction enzyme. It creates blunt ends The nucleic acid sequence where the enzyme cuts is GATATC, which, as the complementary sequence is CTATAG, has rotational symmetry.
• EcoRV - 150 μl
• 10X Reaction Buffer - 1.0ml
-20 °C (Blue/Dry Ice)
24 Months
Not Regulated for Transport (Non-Haz)
38229090 (GST 12%)
