Prices are indicative and are subject to change
Creamish tan
Free flowing, homogeneous powder
30.00 gm/liter
Clear to very slightly opalescent
7.3 ± 0.2
Clear to very slightly opalescent
Medium amber
Inoculate (isolated colony) the tubes by stabbing the butt to the center through 2/3 rds of the medium with the following and incubate at 37°C for 18 -24 hours
After 24 hours of incubation observe the tubes for growth,motility,hydrogen sulphide (H2S) production and indole production by addition of 3 - 4 drops of Kovacs reagent
Escherichia coli ATCC 25922
Growth : Good
Motility : (+)
Indole : (+)
H2S : (-)
Salmonella typhimurium ATCC 14028
Growth : Good
Motility : (+)
Indole : (-)
H2S : (+)
Salmonella typhi ATCC 6539
Growth : Good
Motility : (+)
Indole : (-)
H2S : (+)
Shigella flexneri ATCC 12022
Growth : Good
Motility : (-)
Indole : (-)
H2S : (-)
Klebsiella pneumoniae ATCC 13883
Growth : Good
Motility : (-)
Indole : (-)
H2S : (-)
(+) Motiliy : is observed as diffused growth outward from the stab line or turbidity of the medium.
(+) H2S : is observed by blackening of the medium or along the stab line.
(+) Indole : is indiacted by a red colour after addition of Kovacs Reagent.
Used for the differentiation of members of the Enterobacteriaceae, based onH2S production, indole production and motility.
gm/lt.
20.00
6.71
3.00
0.20
0.025
1. Add 30.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense the medium into test tubes to a depth of 3 inches preferably.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
8 to 25°C (Cool & Dry Area)
36 Months
Not Regulated for Transport (Non-Haz)
38210000 (GST 18%)
38210000 (GST 18%)
