Pack Size

Price

250 Units (Weiss)
74.75
1000 Units (Weiss)
127.13

Prices are indicative and are subject to change

Appearance (Form)

Liquid

Appearance (Colour)

Colourless

Appearance (Clarity)

Clear

Activity

Enzyme is tested in ligation of Lambda DNa digests at 16°C within one hour.

Description :


T4 DNA ligase is an ATP-dependent recombinant enzyme, isolated from Escherichia coli, used for DNA cloning. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. It will join both blunt-ended and cohesive-ended restriction fragments of DNA as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Applications :


Joins DNA molecules with compatible cohesive termini.
Joins blunt-ended double stranded DNA molecules to one another or to synthetic linkers.
Gene cloning - recombination of vector and insert.

Includes :


250 U (Weiss):
T4 DNA Ligase RC - 50 μl
10X Ligation Buffer - 1.5 ml

1000 U(Weiss):
T4 DNA Ligase RC - 200 μl
10X Ligation Buffer - 2 X 1.5 ml

5000 U(Weiss):
T4 DNA Ligase RC - 1000 μl
10X Ligation Buffer - 5 X 1.5 ml

Storage

-20 °C (Blue/Dry Ice)

Shelf Life

24 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


1000 Units (Weiss)

38229090 (GST 12%)

250 Units (Weiss)

38229090 (GST 12%)

Product Datasheet

Sample COA

Certificate Of Analysis