Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
50.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar and 1.0% gelatin gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 18-22°C for 40-48 hours. Gelatin liquefaction is observed by flooding the plates with standard ammonium sulphate solution.
Organism
Escherichia coli
ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Gelatin liquefaction : (- ve)
Organism
Bacillus cereus
ATCC 10876
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Gelatin liquefaction : (+)ve
Organism
Pseudomonas aeruginosa
ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Gelatin liquefaction :(+)ve
Organism
Proteus vulgaris
ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Gelatin liquefaction : (-)ve
Organism
Enterococcus faecalis
ATCC 29212
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Gelatin liquefaction : (+)ve
Organism
Aeromonas hydrophila
ATCC 7966
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Gelatin liquefaction : (+)ve
Key
(+)ve = clear zone around the colony
(-)ve = no clear zone around the colony
Applications
Used in water and waste water testing for the determination of total microbial count and for the detection of gelatin liquefying microorganisms according to the German methods for the examination of water and the German drinking water regulations (1990).
Composition
Ingredients
gms/lt.
Meat peptone
10.00
Meat extract
10.00
Sodium chloride
5.00
Gelatin
10.00
Agar
15.00
Directions
1. Add 50.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Dispense into sterile petri plates.
