Appearance (Colour)
Light yellow to orange
Appearance (Form)
Free flowing,homogeneous powder
Solubility
44.86 gms/lit.
pH
(at 25°C) 7.2±0.2
Solubility before autoclaving (Colour)
Red
Solubility before autoclaving (Clarity)
Slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Red
Gel strength
Firm ,comparable with 1.2% agar gel.
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Cultural Response
Inoculate and incubate at 25-30°Cfor 48-72 hours.
(Cultural characteristics observed on addition of 100 lU/ml Penicillin G, sodium salt and 0 mcg/ml Pimaricin)
Organism
Aeromonas hydrophila ATCC 7966
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Starch Hydrolysis: positive reaction,acid production,yellow colour
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Recovery Rate : >=50.0%
Starch Hydrolysis: negative reaction,no acid production
Applications
Used for detection of Pseudomonas and Aeromonas in foodstuffs, wastewater and equipment in food industry.
Composition
Ingredients
gms/lt.
L-Glutamate, sodium
10.00
Starch, soluble
20.00
Potassium dihydrogen phosphate
2.00
Magnesium sulphate
0.50
Phenol Red
0.36
Agar
12.00
Directions
1. Add 44.86 grams in 1000 ml distilled/purified water.
2. Gently heat and bring to boiling to dissolve the medium completely.
3.Autoclave at 15 psi pressure at 121°C for 15 minutes.
4.Cool to 45-50°C and add 100 lU/ml Penicillin G, sodium salt and if desired,add 10 mcg/ml Pimaricin.
5. Mix well and dispense as desired.
