Appearance (Colour)
Light tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
38.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent solution
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent solution.
Prepared Medium Appearance after autoclaving (Colour)
Medium amber
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-48 hours
Organism
Clostridium perfringens ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacteroides fragilis ATCC 23745
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium sporogenes ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
Applications
Used for the cultivation and enumeration of Clostridia in pharmaceutical testing according to harmonized methods.
Composition
Ingredients
gm/lt.
Peptone
10.00
Beef extract (ex. buffalo)
10.00
Sodium acetate
3.00
Yeast extract
3.00
Soluble starch
1.00
Dextrose
5.00
Cysteine hydrochloride
0.50
Sodium chloride
5.00
Agar
0.50
Directions
1. Add 38.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Dispense into test tubes, as desired.
