Appearance (Colour)
Light pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
64.62 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Pinkish red
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth: Good, Slant: A, Butt: A, Gas: +, H2S: -
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth: Good, Slant: A, Butt: A, Gas: +, H2S: -
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth: Good, Slant: K, Butt: A, Gas: +, H2S: +
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth: Good, Slant: A, Butt: A, Gas: +, H2S: -
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth: Good, Slant: K, Butt: K, Gas: -, H2S: -
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth: Good, Slant: K, Butt: A, Gas: -, H2S: +
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth: Good, Slant: K, Butt: A, Gas: -, H2S: -
Key
A = acidic, yellow; K = alkaline, no change; Gas = (+) splitting and cracking of the medium, Gas = (-) no splits, cracks or bubbles, H2S = (+) black precipitate; H2S = (-) no black precipitate
Applications
Used for the differentiation of members of Enterobacteriaceae on the basis of fermenting of glucose, lactose, sucrose and H2S production.
Composition
Ingredients
gm/lt.
Peptone
20.00
Beef extract (ex. buffalo)
3.00
Yeast extract
3.00
Sodium chloride
5.00
Glucose
1.00
Lactose
10.00
Sucrose
10.00
Ferric citrate
0.30
Sodium thiosulphate
0.30
Phenol Red
0.025
Agar
12.00
Directions
1. Add 64.625 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Prepare slants and butts.
