Appearance (Colour)
Off-white to pale brown
Appearance (Form)
Crystalline powder
Activity
min. 20 DEA units/mg solids
min. 25 DEA units/mg protein
Protein content
min. 80%
Unit Definition
One unit will hydrolyze 1.0 micromole of p-nitrophenyl phosphate per minute at pH 9.8 at 37 °C using diethanolamine buffer. ( 3 DEA units = 1 Glycine unit)
Enzyme solution for activity determination
Prepared in 0.1 M Diethanolamine buffer containing 0.25 mM magnesium chloride, pH 9.5.
