Appearance (Colour)
Light beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
58.00 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm comparable with 2.0 % agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent, as the medium cools, becomes greenish due to aeration
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 48 -72 hours
Organism
Clostridium perfringens ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=50.0%
Organism
Bacteroides fragilis ATCC 25285
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=50.0%
Organism
Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=50.0%
Applications
For the cultivation of anaerobic bacteria, especially Clostridium species.
Composition
Ingredients
gms/lt.
Agar
20.00
Tryptone
20.00
Dextrose
10.00
Sodium chloride
5.00
Sodium thioglycollate
2.00
Sodium formaldehyde sulfoxylate
1.00
Methylene Blue
0.002
Directions
1. Add 58.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
