Appearance (Colour)
White to off-white
Appearance (Form)
Crystalline powder
Loss on drying
max. 5%
Activity
min. 1:2500 (Egg Albumin digesting power)
min. 0.6 Anson units/mg
Unit Definition
1:2500- One gram of pepsin will digest 2500 grams of coagulated egg albumin.
Anson unit: One unit will produce ΔA at 280 nm of 0.001 per minute at pH 2.0 and 37 °C, measured as TCA soluble products using hemoglobin as a substrate.
Enzyme solution for activity determination
Prepared in 0.1 N HCl
Notes
Pepsin 1:3000 is equivalent to 3000 FCC units/mg.
3000 FCC U/mg = 0.5 U/mg USP = 0.5 U/mg Ph Eur = 0.5 U/mg FIP
