Composition
Reinforced Medium for Clostridia,Harmonized - 11999
Columbia Agar Base,Harmonized - 21493
Reinforced Medium for Clostridia,Harmonized - 11999
Dehydrated Appearance
Light tan, free flowing, homogeneous powder
Solubility
38.00 gm/liter
Prepared Medium Appearance
Medium amber, clear to very slightly opalescent solution.
pH (25°C)
6.8 ± 0.2
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours
Organism
Clostridium perfringens ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium sporogenes ATCC 19404
Inoculum (cfu) 10-100
Growth : Good
Columbia Agar Base,Harmonized - 21493
Dehydrated Appearance
Light yellow, free flowing, homogeneous powder
Solubility
44.00 gm/liter
Prepared Medium Appearance
Cherry red, opaque (w/ addition of blood)
pH (25°C)
7.3 ± 0.2
Cultural Response
Inoculate and incubate at 37°C for 24 - 48hours with the following:
Organism
Clostridium perfringens ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Clostridium sporogenes ATCC 19404
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Applications
Used for isolation, identification, cultivation of Clostridium species.
