Appearance (Colour)
White to off-white
Appearance (Form)
Free flowing, homogeneous powder
Solubility
16.09 gms/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Colourless
Cultural Response
Colony characteristics observed after recovery on Soybean Casein Digest Agar (33291) after an incubation at 30-35°C for 18-24 hours for bacteria and Sabouraud Dextrose Agar (28055) at 30-35°C for 24-48 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Recovey within 2 hours incubation : no decrease in colony count
Recovery within 4 hours incubation : no decrease in colony count
Recovery within 24 hours incubation : no decrease in colony count
Organism
Escherichia coli ATCC 8739
Inoculum (cfu) 10-100
Recovey within 2 hours incubation : no decrease in colony count
Recovery within 4 hours incubation : no decrease in colony count
Recovery within 24 hours incubation : no decrease in colony count
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Recovey within 2 hours incubation : no decrease in colony count
Recovery within 4 hours incubation : no decrease in colony count
Recovery within 24 hours incubation : no decrease in colony count
Organism
Staphylococcus aureus ATCC 6538
Inoculum (cfu) 10-100
Recovey within 2 hours incubation : no decrease in colony count
Recovery within 4 hours incubation : no decrease in colony count
Recovery within 24 hours incubation : no decrease in colony count
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Recovey within 2 hours incubation : no decrease in colony count
Recovery within 4 hours incubation : no decrease in colony count
Recovery within 24 hours incubation : no decrease in colony count
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Recovey within 2 hours incubation : no decrease in colony count
Recovery within 4 hours incubation : no decrease in colony count
Recovery within 24 hours incubation : no decrease in colony count
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Recovey within 2 hours incubation : no decrease in colony count
Recovery within 4 hours incubation : no decrease in colony count
Recovery within 24 hours incubation : no decrease in colony count
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Recovey within 2 hours incubation : no decrease in colony count
Recovery within 4 hours incubation : no decrease in colony count
Recovery within 24 hours incubation : no decrease in colony count
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Recovey within 2 hours incubation : no decrease in colony count
Recovery within 4 hours incubation : no decrease in colony count
Recovery within 24 hours incubation : no decrease in colony count
Applications
An isotonic agent recommended for use as a diluent in performing microbial examination of non-sterile products.
Composition
Ingredients
gm/lt.
Peptone
1.0
Potassium Dihydrogen phosphate
3.56
Disodium hydrogen phosphate
7.23
Sodium chloride
4.30
Directions
1. Add 16.09 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. If desired ,add 0.1 to 1%w/v Polysorbate 20 or 80.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
