Appearance (Colour)
Cream colour
Appearance (Form)
Free flowing, homogeneous powder
Solubility
42.25 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medim amber
Cultural Response
Inoculate and incubate at 35°C - 37°C for 24- 48 hours
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth- Good
Organism
Lactobacillus leichmanii ATCC 4797
Inoculum (cfu) 10-100
Growth- Good
Organism
Lactobacillus plantarum ATCC 8014
Inoculum (cfu) 10-100
Growth- Good
Organism
Streptococcus thermophilus ATCC 14485
Inoculum (cfu) 10-100
Growth- Good
Applications
For improved growth of lactic streptococci and their bacteriophages.
Composition
Ingredients
gm/lt.
Peptic digest of soyabean meal
5.00
Casein enzymatic hydrolysate
5.00
Beef extract (ex.buffalo)
5.00
Ascorbic acid
0.50
Yeast extract
2.50
Magnesium sulphate
0.25
Lactose
5.00
Disodium beta glycerophosphate
19.00
Directions
1. Add 42.25 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
