Solubility
40.00 gm/liter.
Solubility before autoclaving (Clarity)
Very slightly opalescent.
Gel strength
Firm, comparable with 1.4% agar gel.
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent.
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber.
Cultural Response
Inoculate and Incubate at 35° - 37°C for 18-24 hours.
Organism
Streptococcus pyogens ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery w/addition of blood : >=70.0%
Haemolysis : Beta
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery w/addition of blood : >=70.0%
Haemolysis : Beta
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery w/addition of blood : >=70.0%
Haemolysis : no haemolysis
Organism
Streptococcus pneumoniae ATCC 6305
Inoculum (cfu) 10-100
Growth : Good
Recovery w/addition of blood : >=70.0%
Haemolysis : Alpha haemolysis.
Applications
Recommended for the cultivation of fastidious microorganisms and detection of their hemolytic activity.
Composition
Ingredients
gms/lt.
Enzymatic digest of casein
14.50
Sodium chloride
5.00
Soyapeptone
5.00
Growth factor
1.50
Agar
14.00
Directions
1. Add 40.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Cool to 45- 50°C.
6. Aspetically add 50ml sterile defibrinated blood and mix well.
