Appearance (Colour)
White with pink tint
Appearance (Form)
Free flowing, homogeneous powder
Solubility
3.27 gm/liter
Solubility before autoclaving (Clarity)
White precipitate
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear supernatant over yellow-orange precipitate
Prepared Medium Appearance after autoclaving (Colour)
Colourless to very light amber
Cultural Response
Inoculate and incubate at 25-30°C for upto 7 days
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth (Plain) : Poor
Recovery w/hydrocarbons : Good
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth (Plain) : Poor
Recovery w/hydrocarbons : Good
Applications
For examining fuels for microbial contamination and for studying hydrocarbon utilization by microorganisms.
Composition
Ingredients
gm/lt.
Magnesium sulphate
0.20
Calcium chloride anhydrous
0.02
Monopotassium phosphate
1.00
Dipotassium phosphate
1.00
Ammonium nitrate
1.00
Ferric chloride
0.05
Directions
1. Add 3.27 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into test tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
