Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
17.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good,
MR test : +,
VP test : -
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good,
MR test : -,
VP test : +
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good,
MR test : -,
VP test : +
Key
Methyl red test :
Positive (+): bright red colour
Negative (-): Yellow-orange colour
Voges-Proskauer test :
Positive (+) : red colour
Negative (-): no red colour
Applications
For the differentiation of bacteria based on acid production (Methyl Red test) and acetoin production (Voges-Proskauer reaction).
Composition
Ingredients
gm/lt.
Pancreatic digest of casein
3.50
Dextrose
5.00
Peptone
3.50
Dihydrogen potassium phosphate
5.00
Directions
1. Add 17.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
