Appearance (Colour)
Pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
52.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Orange-brown
Cultural Response
Inoculate and incubate at 37°C for 24-48 hours
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate: <=50%, Colony colour : Red
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : None-poor, Recovery Rate: <=10%, Colony colour : Red
Organism
Escherichia coli ATCC 25922
Inoculum
Growth: Inhibited, Recovery rate : >= 103
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate: >=50%, Colony colour : Red
Applications
For the selective isolation of Salmonellae from faeces, water, food and dairy products.
Composition
Ingredients
gm/lt.
Peptone
10.00
Beef extract (ex.buffalo)
5.00
Agar
12.00
Yeast extract
3.00
Lactose
10.00
Monosodium phosphate
0.60
Disodium phosphate
1.00
Sucrose
10.00
Brilliant Green
0.0047
Phenol Red
0.09
Directions
1. Add 52.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave.
4. If desired, aseptically add 10.0 ml of Sulpha supplement (34050)
