Appearance (Colour)
White, fine powder
Solubility
Completely soluble in 0.01N Sodium hydroxide
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24-48 hours (Aseptically add the rehydrated contents of vial as per direction) Growth on Baird-Parker Agar, Base (24562)
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Colony colour: Grey-black, shiny
Lecithinase halo : Positive
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Poor to Good
Colony colour: Black
Lecithinase halo : Negative.
Organism
Bacillus subtilis ATCC 6633
Inoculum
Growth : None to poor
Colony colour: Brown
Lecithinase halo : Negative
Organism
Proteus mirabilis ATCC 25933
Inoculum
Growth : Inhibited
Colony colour: No growth
Lecithinase halo : Negative
Applications
Used for suppressing the growth of Proteus species on Baird Parker Agar Base.
Composition
Sulphamethazine
50.00 mg/vial
Directions
1. Aseptically add 5ml of sterile 0.01N Sodium Hydroxide to 1 vial.
2. Mix well and aseptically add to sterile cooled media at 45-50°C and pour into sterile petriplates.
3. Add 5.0 ml of supplement to 1000 ml of Baird-Parker Agar, Base (24562), (50222).
