Appearance (Colour)
Brownish yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
54.20 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Medium amber
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24-48 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good, Tellurite Reaction : (+)
Organism
Escherichia coli ATCC 25922
Inoculum
>=103,Growth : Inhibited, Tellurite Reaction : (-)
Organism
Micrococcus luteus ATCC 10240
Inoculum
>=103,Growth : Inhibited, Tellurite Reaction : (-)
Key
Tellurite Reaction (+) : Positive, blackening at the bottom of tubes / general blackening of the medium
Tellurite Reaction (-) : No blackening of medium
Applications
Used for the cultivation and enrichment of Staphylococcus aureus from foods.
Composition
Ingredients
gm/lt.
Tryptone
10.00
Beef extract (ex.buffalo)
5.00
Yeast extract
5.00
Mannitol
20.00
Sodium chloride
5.00
Lithium chloride
5.00
Glycine
1.20
Sodium pyruvate
3.00
Directions
1. Add 54.20 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense 19 ml medium into each test tube.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
6. Cool the tubes at room temperature.
7. Aseptically add 0.3 ml of 3.5% Potassium tellurite solution(99482) to each tube.
