Appearance (Colour)
Beige
Appearance (Form)
Free,flowing,homogeneous powder.
Solubility
43.41 gms/lit.
pH (25°C)
7.4 -7.8
Gel strength
Firm,comparable with 1.5% agar gel
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Cultural Response: Inoculate and incubate at 37° C for 18-24hrs
with addition of blood and supplements under anaerobic conditions.
Organism
Bacteroides fragilis ATCC 25285
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Clostridium perfringens ATCC 12924
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Escherichia coli ATCC 25922
Inoculum
>=10³
Growth : Inhibited
Applications
Used for the enumeration of various aerobic and anaerobic bacterial species present in the gastrointestinal tract.
Composition
Ingredients
gm/lt.
Enzymatic Digest of Casein
5.67
Enzymatic Digest of Animal Tissue
5.00
Soya peptone
1.00
Yeast extract
5.00
Dextrose
5.83
Sodium chloride
1.67
Tris (hydroxymethyl) aminomethane
3.00
Hemin
0.01
L-Cystine
0.40
Dipotassium hydrogen phosphate
0.83
Agar
15.00
Directions
1. Suspend 43.41 gms of powder in 950 ml distilled/purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 15 lbs pressure at 121 °C for 15 minutes.
4. Cool to 45-50°C.
5. If desired,add 5.0%v/v sterile defibrinated sheep blood.
6. If desired,add rehydrated contents of Vitamin k1 supplement (34326) and CNA supplement (64078).
7. Mix well and dispense as desired.
