Appearance (Colour)
Cream to yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
21.24 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent gel
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent gel
Prepared Medium Appearance after autoclaving (Colour)
Light yellow coloured
Cultural Response
Inoculate and incubate at 35-37°C for 24-72 hours
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Applications
For the cultivation and maintenance of heterotrophic bacteria from potable waters.
Composition
Ingredients
gm/lt.
Agar
15.00
Yeast extract
1.00
Acid hydrolysate of casein
1.00
Dextrose
1.00
Soluble starch
1.00
K2HPO4
0.60
Sodium pyruvate
0.60
Pancreatic digest of casein
0.50
Peptic digest of animal tissue
0.50
MgSO4 anhydrous
0.048
Directions
1. Add 21.24 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
