Appearance (Colour)
Light cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
30.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours for bacteria and at 25°C for fungal * organisms
Organism
Escherichia coli
ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacillus subtilis
ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus
ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacteroides vulgatus
ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes
ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Organism
Pseudomonas aeruginosa
ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Organism
Salmonella typhimurium
ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Organism
Candida albicans*
ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Organism
Micrococcus luteus
ATCC 9341
Inoculum (cfu) 10-100
Growth : Good
Applications
For the cultivation of a variety of microorganisms.
Composition
Ingredients
gm/lt.
Veg. Hydrolysate
17.00
Sodium chloride
5.00
Veg. Peptone
3.00
Dipotassium phosphate
2.50
Dextrose
2.50
Directions
1. Add 30.00 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
