Appearance (Colour)
White to off white
Appearance (Form)
Free flowing homogeneous powder
Solubility (Colour) 1% aq. solution
Soluble in distilled water, insoluble in alcohol and ether
Solubility before autoclaving (Clarity)
(1%) Clear and free from extraneous matter
pH (1% aq. solution)
7.00 - 9.00
Solubility after autoclaving (Clarity)
(1%) Clear and free from extraneous matter
Solubility after autoclaving (Colour)
1% aqueous solution is light straw colour
Cultural Response: Inoculate and incubate at 37° C for 18-24hrs
Incubate at 37°C for 18-24 hours by preparing MacConkey Agar No. 3
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth- Good, Colony colour- Pink to red with bile precipitate
Organism
Enterococcus aerogens ATCC 13048
Inoculum (cfu) 10-100
Growth- Good, Colony colour- Pink to red
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth- Good, Colony colour- Colourless
Organism
Salmonella paratyphi B ATCC 8759
Inoculum (cfu) 10-100
Growth- Good, Colony colour- Colourless
Deoxycholic acid-sodium salt
~ 45 -55%
Cholic acid-sodium salt
~ 45 -55%
Loss on drying
< 5.0 %
Applications
Bile salts No. 3 is a refined bile salt for use as a selective inhibitory agent in bacteriological culture media. It consist of a specially modified fraction of bile acid salts (comprising mainly of sodium cholate and sodium deoxycholate).
