Appearance (Form)
Liquid
DNA Digestion
DNA was digested with the two restriction enzymes at 37°C in a period of one hour
DNA degradation
DNA was incubated at 37°C for 24 hours
Description
The aim of the experiment is to cleave a given DNA specifically at particular sequences using restriction enzyme. This simple but powerful technique has been the mainstay in experimental molecular biology of DNA to generate fragments of defined sizes and having proper ends for cloning, release cloned fragments of DNA, characterizing a given DNA by restriction mapping and use such a map to differentiate between closely and distantly related genes or DNA.
In this experiment, use of restriction enzymes, in restriction mapping of lambda DNA by digestion with two enzymes Hind III and EcoRI is demonstrated.
Includes
| Components | 10 Experiments | 50 Experiments |
|---|
| Lambda DNA(250 ng/mcl) | 160 mcl | 1.0ml |
| Agarose | 1 gm | 1.5 gm |
| 50 X TAE stock Buffer | 25 ml | 50 ml |
| Ethidium Bromide | 30 mcl | 100 mcl |
| 6 X Gel loading Dye | 200 mcl | 500 mcl |
| 10 X Assay buffer for Hind III | 100 mcl | 250 mcl |
| 10 X Assay buffer for EcoR I | 100 mcl | 250 mcl |
| Hi - Range marker | 60 mcl | 150 mcl |
| Sterile Water | 500 mcl | 1.5 ml |
| EcoRI (1U/3mcl) | 100 mcl | 200 mcl |
| Hind III (1U/3mcl) | 100 mcl | 200 mcl |
