Appearance (Colour)
Cream
Appearance (Form)
Free flowing ,homogeneous fine powder
Solubility (Colour) 2% aq. solution
Light to medium amber, clear
pH (25°C)
6.2 - 7.2
Stability (After autoclaving)
Light to medium amber, clear
Loss on drying
< =5.0%
Nitrogen content (N)
12.0-13.5%
NaCl
< 5.0%
Ash
< 15%
Total Aerobic microbial count
(By Plate Count Method) : < =10,000 cfu/gm
Indole Production (0.1%)
Escherichia coli ATCC 25922
Growth : Good, Result : Positive
Fermentable carbohydrates (2%)
Escherichia coli ATCC 25922
Growth : Good, Result : Negative
Acetylmethylcarbinol Production
Enterobacter aerogenes ATCC13048
Growth : Good, Result : Positive
Hydrogen Sulphide Production (1.0%)
Salmonella typhimurium ATCC 14028
Growth : Good, Result : Positive
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours with the following organisms
Organism
Staphylococcus aureus ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Applications
Tryptone Type I is an enzymatic digest of milk protein, rich in amino nitrogen source; used in microbiological and fermentation media.
