Appearance (Colour)
Biege to greenish biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
40.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Emerald green
Cultural Response
Inoculate and incubate at 37°C for 18-48 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Gas production : +
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good, Gas production : +
Organism
Bacillus cereus ATCC 10876
Inoculum (cfu) 10-100
Growth : Inhibited, Gas production : -
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Inhibited, Gas production : -
Organism
Enterococcus faecalis ATCC 19433
Inoculum (cfu) 10-100
Growth : Inhibited, Gas production : -
Applications
Recommended medium for detection and confirmation of coliforms from water, wastewater, foods, milk and dairy products.
Composition
Ingredients
gm/lt.
Peptone
10.00
Lactose
10.00
Oxgall
20.00
Brilliant Green
0.0133
Directions
1. Add 40.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Distribute into tubes containing inverted Durham’s tubes
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
