Appearance (Colour)
Light grey
Appearance (Form)
Free flowing, homogeneous powder
Solubility
47.02 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.45% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Purple
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-48 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Mannitol fermentation : Positive reaction, yellow color
Coagulase production : positive reaction, opaque zone around the colonies
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Mannitol fermentation : Negative reaction,purple color
Coagulase production : Negative reaction, no formation of opaque zone around the colonies -
Applications
For the cultivation, differentiation of Staphylococcus aureus from other Staphylococcus species, based on coagulase production and mannitol fermentation.
Composition
Ingredients
gms/lt.
Brain heart infusion
5.00
Tryptone
10.50
Soyatone
3.50
Sodium chloride
3.50
Mannitol
10.00
Bromocresol Purple
0.02
Agar
14.50
Directions
1. Add 47.02 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C and add 7-15% sterile, rabbit plasma to the basal medium.
5. Mix well and pour in sterile petri plates.
