Sisco Research Laboratories
Pvt. Ltd.
Product Datasheet
and Specification
19317

BioLit Gene Amplification Kit - PCR (Teaching)

Part E

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Specifications
Amplification capability
Amplification with good yield is seen with the template and primers in the kit
PCR amplification
Protocol given with kit shows good amplification with amplicons of the sizes ranging from either 600 to 900bp.
Description
Polymerase Chain Reaction (PCR) is an important tool in Biotechnology. PCR rapidly amplifies a specific region of a single DNA molecule in vitro to yield sufficient quantities that can be cloned, sequenced, analyzed by restriction mapping etc. In this kit the gene for E.coli pyrophosphatase is amplified from its template DNA using specific primers in 30 cycles of operation.
Includes
Components10 Experiments25 Experiments
Control (with 36 mcl of PCR master mix)12 vials28 vials
Test (with 1 mcl template)12 vials28 vials
Taq Polymerase (5U/mcl)10 mcl20 mcl
Enzyme Diluent Buffer50 mcl150 mcl
6 X Gel loading Dye120 mcl250 mcl
Agarose0.75 g2.5 g
Ethidium Bromide50 mcl100 mcl
50 X TAE buffer25 ml50 ml
Sterile Water100 mcl250 mcl
DNA marker60 mcl100 mcl
General Information

Storage

-20 °C (Blue/Dry Ice)

Shelf Life

12 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :

10 expt. Kit

38229090 (GST 12%)

25 expt. Kit

38229090 (GST 12%)

Available Packages
Pack Size
10 expt. Kit
Pack Size
25 expt. Kit