Appearance (Colour)
Greenish yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
33.17 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
8.6 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Greenish blue
Cultural Response
Inoculate and incubate at 37°C for 24-48 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : golden yellow
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : blue/colourless
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : yellow
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : blue/colourless
Applications
For the isolation and cultivation of pathogenic Staphylococci.
Composition
Ingredients
gm/lt.
Proteose peptone
5.00
Beef extract (ex.buffalo)
3.00
Agar
15.00
Lactose
10.00
Bromothymol Blue
0.17
Directions
1. Add 33.17 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
