Appearance (Colour)
Light cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
40.00 gm/liter
Solubility before autoclaving (Clarity)
Very slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Applications
For the cultivation and maintenance of a wide variety of microorganisms.
Composition
Ingredients
gm/lt.
Agar
15.00
Pancreatic digest of casein
15.00
Sodium chloride
5.00
Pancreatic digest of soybean meal
5.00
Directions
1. Add 40.00 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
