Appearance (Colour)
Light to medium tan
Appearance (Form)
Free,flowing homogeneous powder
Solubility (Colour) 2% aq. solution
Light to medium amber, clear
pH (25°C)
7.0 ± 0.5 (2.0%)
Stability (After autoclaving)
Light to medium amber, clear
Loss on drying
< = 5.0%
Nitrogen content (N)
9.0 - 10.5%
NaCl
< 5.0%
Ash
<= 22.0%
Total Aerobic microbial count
(By Plate Count Method) : < 10,000 cfu/gm
Cultural Response
Inoculate and incubate at 37°C for 18 -24 hours with the following organisms
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Indole Production (0.1%)
Escherichia coli ATCC 25922
Growth : Good, Result : Positive
Hydrogen Sulphide Production (1.0%)
Salmonella typhimurium ATCC 14028
Growth : Good, Result : Positive
Fermentable carbohydrates (2%)
Escherichia coli ATCC 25922
Growth : Good, Result : Positive
Acetylmethylcarbinol Production
Enterobacter aerogenes ATCC 13048
Growth : Good, Result : Positive
Applications
Papaic digest of soyabean meal phytone, a peptone of plant origin. Contains high concentration of carbohydrates and nitrogen constituents, hence making it suitable for growth of fastidious microorganisms.
