Appearance (Form)
free flowing, homogeneous powder
Solubility
104.55 gm/lit
Solubility before autoclaving (Clarity)
Light to medium amber, clear solution without any precipitate
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear solution without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
2 ml of M-Staphylococcus broth is used to saturate sterile absorbent cotton pads. Inoculate and incubate at 35°C - 37°C for 18-48 hours with the following organisms. Observe membrane for growth and pigment production. Mannitol fermentation can be visualized as yellow colouration by addition of few drops of bromothymol blue to areas from where colonies have been removed.
Organism
Enterococcus faecalis ATCC 29212
Inoculum
Inoculum (cfu): >=10³
Growth: Inhibited
Organism
Streptococcus pyogenes ATCC 19615
Inoculum
Inoculum (cfu): >=10³
Growth: Inhibited
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth: Good
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth: Good
Organism
Escherichia coli ATCC 25922
Inoculum
Inoculum (cfu): >10³
Growth: Inhibited
Applications
Used for detection and isolation of Staphylococci by memebrane filter technique.
Composition
Ingredients
gms/lt.
Casein enzymic hydrolysate
10.00
Yeast extract
2.50
Lactose
2.00
Mannitol
10.00
Dipotassium hydrogen phosphate
5.00
Sodium chloride
75.00
Sodium azide
0.049
Directions
1. Add 104.55 gm powder to 1.0 liter of distilled/ purified water and mix thoroughly.
2. Gently heat to boiling for 5 minutes.
3. Do not autoclave.
