Appearance (Colour)
Cream to yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
42.23 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 2.0% agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light yellow
Cultural Response
Incubate at 35 ± 2°C for 18-24 hours.
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Pigment production : greenish yellow
Organism
Pseudomonas aeruginosa ATCC 17934
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Pigment production : greenish yellow
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Pigment production : greenish yellow
Organism
Burkholderia cepacia ATCC 25609
Inoculum (cfu) 10-100
Growth : Good
Pigment production : No pigment
Applications
For the non-selective isolation, cultivation and pigment production of Pseudomonas species.
Composition
Ingredients
gm/lt.
Proteose peptone
20.00
Dipotassium hydrogen phosphate
1.50
Magnesium sulphate, heptahydrate
1.50
Agar
20.00
Directions
1. Add 42.23 gm powder to distilled/purified water .
2. Bring volume to 1.0 liter containing 15.0 ml glycerol and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Dispense into sterile petri plates.
