Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
104.40 gm/lit
Solubility before autoclaving (Clarity)
slightly opalescent
Solubility before autoclaving (Colour)
Yellow
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Yellow
Gel strength
semi-solid,comparable with 0.4% agar and 8.0% Gelatin
Cultural Response
Inoculate and incubate at 35°C for 18-24 hours with the following:
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth: Good
Motility : (+)ve
H2S : (-) ve
Urease : (-)ve
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth: Good
Motility : (+)ve
H2S : (-) ve
Urease : (-)ve
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth: Good
Motility : (+)ve
H2S : (+) ve
Urease : (-)ve
Urease: -
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth: Good
Motility : (-)ve
H2S : (-) ve
Urease : (-)ve
Organism
Proteus mirabilis* ATCC 25933
Inoculum (cfu) 10-100
Growth: Good
Motility : (±)ve
H2S : (+)ve
Urease : (+)ve
Organism
Shigella sonnei ATCC 25931
Inoculum (cfu) 10-100
Growth : Good
Motilit y : ( -)ve
H2S :(-)ve
Urease : (-)ve
Key
Motility : (+)ve, positive reaction, indicated by growth away from stabline
(-)ve, negative reaction, indicated by growth along stab line
* .is temperature dependent, more pronouced at 20°C and absent at 35°C.
H2S : (+)ve, positive reaction, blackening of medium
H2S : (-)ve, negative reaction, no blackening of medium
Urease : (+)ve, positive reaction, dark pink-red colour of medium
Urease : (-)ve, negative reaction, no change in colour of medium
(+)ve: positive reaction, motile organism / blackening of medium/ reddish purple colouration
Applications
For the determination of bacterial motility and the ability of bacteria to produce H2S from L- Cystine.
Composition
Ingredients
gms/lt.
Gelatin
80.00
Proteose peptone
10.0
Sodium chloride
5.00
Agar
4.00
Beef extract (ex. Buffalo)
3.00
Sodium citrate
2.00
L-Cystine
0.20
Ferric ammonium citrate
0.20
Directions
1. Add 104.40 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense in tubes in 4ml amounts.
4. Autoclave at 10 psi pressure at 115°C for 15 minutes.
5. Allow the tubes to soldify in upright position.
