Appearance (Colour)
Cream to yellow coloured
Appearance (Form)
Free flowing, homogeneous powder
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Solubility
63.05 gm/liter
Gel strength (1.5% gel)
Firm, comparable with 1.5% Agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 35°C - 37°C for 24-48 hours
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : None- poor, Recovery : <=10%, Colony colour : Dark Brown Matt, Lecithinase : (-)
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good, Recovery : =>50%, Colony colour : Grey - Black shiny, Lecithinase : (+)
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth : None- poor, Recovery : <=10%, Colony colour : Brown - Black w/o swarming, Lecithinase : (-)
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : None- poor, Recovery : <=10%, Colony colour : Large Brown -Black, Lecithinase : (-)
Organism
Micrococcus luteus ATCC 10240
Inoculum (cfu) 10-100
Growth : Fair - good, Recovery : <=30- 40%, Colony colour : very small Brown- Black, Lecithinase : (-)
Key
Lecithinase halo : (+) indicates halo around colony
Lecithinase halo : (-) indicates no halo around colony
Applications
Recommended medium for isolation and enumeration of coagulase-positive staphylococci from food and other specimens.
Composition
Ingredients
gm/lt.
Casein enzymic hydrolysate
10.00
Beef extract
5.00
Yeast extract
1.00
Glycine
12.00
Sodium pyruvate
10.00
Lithium chloride
5.00
Agar
20.00
Sulphamethazine
0.05
Directions
1. Add 63.05 gm powder to 950.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-500C.Aseptically add 50 ml egg yolk emulsion and 3 ml of sterile 3.5% Potassium Tellurite solution(99482).
5. Mix well.
